Journal: PLoS ONE
Article Title: New Candidate Biomarkers in the Female Genital Tract to Evaluate Microbicide Toxicity
doi: 10.1371/journal.pone.0110980
Figure Lengend Snippet: Summary of proteins whose levels significantly altered upon N9 or BZK treatment determined by proteomic analysis in mouse vaginal brush and tissue samples.
Article Snippet: Nonoxynol-9 (N9) was purchased from Spectrum Chemicals and Laboratory Products (Gardena, CA); tenofovir was purchased from AK Scientific (Union City, CA); benzalkonium chloride (BZK) and high viscosity sodium carboxymethylcellulose (CMC) were purchased from Sigma Aldrich (Saint Louis, MO); mouse and rabbit apolipoprotein A-I, apolipoprotein C-1, CD166, and rabbit IL-8 ELISA kits were purchased from EIAab (Wuhan, China); mouse fibrinogen ELISA kit was purchased from Abcam (Cambridge, MA); mouse plasminogen ELISA kit was purchased from GenWay (San Diego, CA); mouse IL-8 ELISA kit was purchased from MyBioSource (San Diego, CA); rabbit plasminogen ELISA kit was purchased from Alpco (Salem, NH); rabbit fibrinogen ELISA kit was purchased from Molecular Innovations (Novi, MI); medroxyprogesteron acetate was purchased from Pharmacia (New York, NY); CellTiter-Glo was purchased from Promega (Madison, WI); RNeasy kits were purchased from Qiagen (Valencia, CA); rabbit anti-human olfactomedin 4 (OLFM-4) polyclonal antibody was purchased from Abcam (Cambridge, MA); rabbit anti-human mucin 5B polyclonal antibody was purchased from Bioss USA Antibodies (Woburn, MA); rabbit anti-mouse CD166 was purchased from GeneTex (Irvine, CA); rabbit anti-mouse β-actin was purchased from Abcam (Cambridge, MA); IRDye 800 Goat anti-rabbit IgG was purchased from Li-Cor (Lincoln, NE); mouse anti-human β-actin monoclonal antibody was purchased from Cell Signaling Technology (Danvers, MA); donkey anti-mouse IgG antibody conjugated to IRDye 680 LT and goat anti-rabbit IgG antibody conjugated to IRDye 800CW were purchased from LI-COR biosciences (Lincoln, NE).
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